Bioinformatics Toolkit v2.0

Gene Sequence
Analyzer

DNA sequence analysis, mutation detection, ORF discovery, and genomic insights — built for the lab and the classroom.

— Bases loaded

— GC content

— ORFs found

— Variants detected

// input sequence

Drop FASTA file here or click to browse

.fasta · .fa · .fna · .txt

or paste sequence directly

Initializing pipeline… 0%

Analysis complete ✓ PIPELINE OK

—

Sequence length

—

GC content

—

ORFs (ATG→stop)

—

AT / GC ratio

// colorized sequence (A G C T)

Sequence Summary

Sequence ID —

Length —

GC Content —

AT Content —

Reverse Complement

—

RNA Transcript

—

Protein Translation

—

ORF Table

0 ORFs

#	Start	End	Length	Sequence
No ORFs detected yet.

Mutation Comparison

0 variants

Compare a reference DNA sequence against a sample sequence to detect SNPs, insertions, and deletions.

Reference Sequence

Sample Sequence

#	Position	Reference	Sample	Type
No comparison run yet.

Download & Export

// analysis modules

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Module 01

Sequence Statistics

GC content, nucleotide composition, length distribution, and Shannon entropy for sequence complexity scoring.

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⚡

Module 02

Mutation Detection

Detect SNPs, insertions, and deletions via pairwise alignment. Outputs position-annotated variant tables.

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🔍

Module 03

ORF Finder

Identify open reading frames across all 6 reading frames. Highlight ATG start codons and stop codon positions.

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🔬

Module 04

Protein Translation

Translate DNA to amino acid chains using standard codon tables. Export in FASTA or single-letter notation.